1.Study Group

alain tenenbaum

N= 29 Wistar rats
Weight mean : 421g

Origin of animals : Biotério Central of Campus of Ribeirão Preto of São Paulo University
Maintenance : Experimental Pathology Lab of Pathology Department on Medicine School of Ribeirão Preto – USP
Animals keeping : polypropylen boxes
Animals food : basic diet of lab and water
Light conditions : natural light/dark cycles under controlled basic conditions
Experimentators : A.Tenenbaum,R.Mené,Kim
PLEASE GO FURTHER BELOW AND CLICK ON EACH CHAPTER TO SEE ALL DETAILS

2.Animals Sacrifice and Histologic Samples

microtome

The mice were euthanized in CO2 chambers.

  • Incision of the skin with a blade nº 23
  • Finding and isolation of the pretibial muscle using Metzenbaum scissors
  • section of tendons with a Mayo scissors.
  • Fixation of Portions of the muscles on sections of cork oak
  • Embedding using 50% formaldehyde solution - 10% buffered and 50% of pure paraffin for conventional optical microscopic study.
  • Staining Sections as routine histological process.
  • Microtome used : Leika RM 2155
  • Coloration of muscle slices with

-hematoxilin-eosin
-Masson´s trichrome
-and red picro-sirius.

  • Histologic examination was realized with a conventional microscope (Olympus BH-2).
  • Observation under polarized lampe
  • Photographic documentation was realized with a Leika DMR microscope joined to a digital camera Leika CD 300F and compatible PC.

3.Material

boxes rats   6 boxes with 5 rats/ box 

  • Animals were divided in 6 boxes, so that boxes from number 1 to 5 had 5 rats and box number 6 had 4 rats 
  • All animals were submitted to a walking evaluation by footprint impression on special paper , and previous immersion of back legs on a soap solution. 
  • Box 1 had the control group, that received the application of 0,1 ml saline solution ( NaCl 0,9%) on the right pretibial muscle. 
  • The mice of boxes 2 to 5 received 0,1 ml of Endopeels Carbolic Acid  on the same muscle as control group.

  • Box 6 was constituted of 4 rats, and each one received 0,5 ml of Endopeels carbolic acid injected on the subcutaneous layer. 

  • All the animals were submitted to walking evaluation before and after their respective injections.

4.Methods:Animals are sacrified after 10 days, 1 month, 3 months, 7 months

 

Box Number Number of Rats Product Injected Quantity Where Injected How Injected Days of Sacrifice
1 5 NaCl 0.9% 0.1 ml right pretibial muscle IM 10-30-90-120-205
2 5 Endopeel 0.1 ml right pretibial muscle IM 10-30-90-120-205
3 5 Endopeel 0.1 ml right pretibial muscle IM 10-30-90-120-205
4 5 Endopeel 0.1 ml right pretibial muscle IM 10-30-90-120-205
5 5 Endopeel 0.1 ml right pretibial muscle IM 10-30-90-120-205
6 4 Endopeel 0.5 ml above the right pretibial muscle SC     30-90-120-205

5.Control (NaCl 0.9%)

CONTROL Left Pretibial Right Pretibial after 0.1 ml NaCl 0.9% IM
  left leg right leg
    Nothing to declare for saline solution injection

We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of this site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

  I accept cookies from this site.
EU Cookie Directive plugin by www.channeldigital.co.uk